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Changed mitochondrial mass in PPARα –/– (PKO) mouse brain endothelial cells. A and B: Representative Western blot analyses for translocase of outer mitochondrial membrane 20 (TOMM20), <t>peroxisome</t> <t>proliferator-activated</t> <t>receptor</t> <t>γ</t> <t>coactivator</t> <t>1α</t> (PGC-1α), and β-actin and densitometry quantification. Data are expressed as means ± SEM ( B ). n = 3 ( B ). ∗ P < 0.05 ( t- test). BSA, bovine serum albumin; NS, no significant difference; Pal, palmitate; WT, wild type.
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Changed mitochondrial mass in PPARα –/– (PKO) mouse brain endothelial cells. A and B: Representative Western blot analyses for translocase of outer mitochondrial membrane 20 (TOMM20), <t>peroxisome</t> <t>proliferator-activated</t> <t>receptor</t> <t>γ</t> <t>coactivator</t> <t>1α</t> (PGC-1α), and β-actin and densitometry quantification. Data are expressed as means ± SEM ( B ). n = 3 ( B ). ∗ P < 0.05 ( t- test). BSA, bovine serum albumin; NS, no significant difference; Pal, palmitate; WT, wild type.
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Changed mitochondrial mass in PPARα –/– (PKO) mouse brain endothelial cells. A and B: Representative Western blot analyses for translocase of outer mitochondrial membrane 20 (TOMM20), <t>peroxisome</t> <t>proliferator-activated</t> <t>receptor</t> <t>γ</t> <t>coactivator</t> <t>1α</t> (PGC-1α), and β-actin and densitometry quantification. Data are expressed as means ± SEM ( B ). n = 3 ( B ). ∗ P < 0.05 ( t- test). BSA, bovine serum albumin; NS, no significant difference; Pal, palmitate; WT, wild type.
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Changed mitochondrial mass in PPARα –/– (PKO) mouse brain endothelial cells. A and B: Representative Western blot analyses for translocase of outer mitochondrial membrane 20 (TOMM20), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), and β-actin and densitometry quantification. Data are expressed as means ± SEM ( B ). n = 3 ( B ). ∗ P < 0.05 ( t- test). BSA, bovine serum albumin; NS, no significant difference; Pal, palmitate; WT, wild type.

Journal: The American Journal of Pathology

Article Title: Peroxisome Proliferator-Activated Receptor α Deficiency Induces Vascular Pathologies through Endothelial Senescence in Diabetic Retinopathy

doi: 10.1016/j.ajpath.2025.12.006

Figure Lengend Snippet: Changed mitochondrial mass in PPARα –/– (PKO) mouse brain endothelial cells. A and B: Representative Western blot analyses for translocase of outer mitochondrial membrane 20 (TOMM20), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), and β-actin and densitometry quantification. Data are expressed as means ± SEM ( B ). n = 3 ( B ). ∗ P < 0.05 ( t- test). BSA, bovine serum albumin; NS, no significant difference; Pal, palmitate; WT, wild type.

Article Snippet: The primary antibodies included antibodies against PPARα (Thermo Fisher Scientific; catalog number MA1822; 1:1000), translocase of outer mitochondrial membrane 20 (TOMM20; Abcam, Waltham, MA; catalog number ab186735; 1:1000), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α; Novus, Centennial, CO; catalog number NBP1-04676; 1:1000), p21CIP1/WAF1 (P21; Cell Signaling, Danvers, MA; catalog number 37543; 1:1000), 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2; Abcam; catalog number ab137043; 1:1000), occludin (Thermo Fisher Scientific; catalog number 71-1500; 1:1000), and β-actin (Santa Cruz Biotechnology, Dallas, TX; catalog number sc-47778HRP; 1:1000).

Techniques: Western Blot, Membrane